Figure 5

Effects of SA-2 on the SMC viability (a) and migration (b) under oxidative stress conditions. Cells were seeded and allowed to attach on tissue culture plates. For the migration study, micropipette tips were used to make scratch lines on wells, followed by washing with PBS and imaging for initial gap distances. Compound SA-2 at different concentrations, SIN-1 (50 µM), SA-3 (50 µM), or SA-5 (50 µM) was added to cell samples, stressed with H ₂ O ₂ and incubated for 24 hours. Controls were cells not exposed to H₂O₂ or any treatment reagent. N/T samples were cells exposed to H₂O₂ without any test compound. Cell viability was quantified with MTS assays while cell migration was imaged and analyzed for final gap distances via ImageJ. Results were then processed on SigmaPlot for statistical analysis using ANOVA followed by post hoc comparisons. Results are presented as mean values ± SEM. Stars indicate significant differences (P < 0.05; n = 4) with respect to N/T.