Figure 2 | Scientific Reports

Figure 2

From: Crosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves

Figure 2

Validation of differential expression patterns under drought for two candidate genes using GUS reporter system and qRT-PCR. Results of qRT-PCR analyses: (a) drought-inducible GUS expression of LOC-Os05g07810 by promoter trap system, and its expression level under drought stress and diurnal rhythm relative to OsUbi5; and (b) drought-inducible GUS expression of LOC_Os10g42610 by promoter-GUS vector system, and its expression level under drought stress and diurnal rhythm relative to OsUbi5. D0, untreated leaf sample; M3, MOCK (Control) monitored for 3 h after drought stress; M6, MOCK after 6 h; M12, MOCK after 12 h; D3, leaf sample under drought for 3 h; D6, leaf sample under drought for 6 h; D12, leaf sample under drought for 12 h. For analyses of diurnal expression: EN, leaf sample collected at end of night; Mid-D, mid-day; ED, end of day; and Mid-N, middle of night. OsUbi5 was used as internal control for qRT-PCR analysis. Error bars indicate standard errors of 3 replicates. *, **, ***, and ****: values are significantly different from each other at P ≤ 0.05, P ≤ 0.01, P ≤ 0.001, and P ≤ 0.0001, respectively.

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