Figure 2
From: Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue
Co-transplantation of ovarian cortical strips with ExECs improves viability and preserves the follicular pool. (a) Scheme of the experimental design. Frozen-thawed human ovarian tissue was encapsulated in a plasma clot, with or without endothelial cells; ExEC-embedded plasma clot, Control plasma clot, respectively. The clots were transplanted into oophorectomized NSG mice and harvested at the end point of the experiment. (b) Human cortical grafts co-transplanted with hUVEC-derived ExECs (green); red blood cells are labeled by TER-119 and boundaries of the graft are outlined in white. (c) The ratio of surviving normal follicles in human ovarian tissue xenografted with and without the mouse or human ExECs in comparison to no cells. Each row represents a replicate originating from the same patient transplanted into the same mouse, serving as its own control. On each side of the graph, the density of the follicles per millimeter squared is indicated. (d) Median percentage of total follicles from transplantation with and without mouse or human ExECs + MAD. *P < 0.05. (e) Median percentage of the fibrotic area from transplantation with and without mouse or human ExECs + MAD. **P < 0.001.
