Figure 5
In-vitro recruitment of LRBA from brain cytosol to Golgi-near endomembranes of COS7 cell ghosts, observed by IF. (A) Recruitment of LRBA is stimulated by GTPγS and antagonized by BFA. (B) GTPγS-recruited LRBA co-localizes with giantin. Both primary antibodies were from rabbit; the giantin antibody was applied after the LRBA primary and secondary antibody, and subsequent blocking with anti-rabbit Fab fragments. The control without giantin antibody confirms the blocking efficiency. (C) GTPγS-recruited LRBA and NBEA co-localize. Using cytosol from LRBA-KO brain, the LRBA signal is selectively lost. Scale bars: (A) 30 µm; (B,C) 15 µm. Cell nuclei, visible at higher exposures, are indicated by dashed outlines.
