Figure 5
Transcriptional variability of antennal developmental transcription factors across six Drosophila species. (A) Boxplot and (B) heatmap plotting the absolute log fold change (ALFC) in the expression of transcription factors known to specify the adult olfactory system15,16,17,20 across all pairwise interspecies comparisons and across all three developmental time points (T1–T3). The boxplot shows combined values for each transcription factor across development, and includes values for the entire antennal transcriptome (Trans) for comparison. Statistical comparisons show results for ANOVA + Tukey’s HSD (*p < 0.05, **p < 0.01, ***p < 0.001, NS = not significant). The post-hoc test compares bab1 AFLC to the other TF. A non-parametric Kruskal-Wallis H test for the boxplot also had p-value < 0.001 (df = 5). In the heatmap, ALFC values shown are the mean ALFC value across development; invalid comparisons (NAs) are white/blank, highly variable genes are highlighted in red, and numbers indicate bootstrap support for the clustering analysis. (C) Convergence index values (see Methods) showing levels of convergence between D. sec. and D. ere. for antennal developmental transcription factors, averaged across development (T1–T3). Positive values indicate convergence while negative values indicate divergence. Error bars indicate S.E.M. (D) Plot of convergence index values versus absolute log fold change (ALFC) values for antennal developmental transcription factors. ALFC values shown are the mean ALFC value across all interspecies pairwise comparisons. The solid line shows a fitted line, with r as Pearson’s correlation coefficient.
