Figure 4
CgMAVS is involved in the antiviral immunity of the oyster and interacts with the CARD domains of CgRIG-I-1. (A) RNA interference of CgMAVS led to increased sensitivity of oysters to OsHV-1 infection. Healthy oysters were injected with PBS (control), CgMAVS-, or GFP-siRNA (negative control) and 72 h later challenged with 106 OsHV-1 virus particles and cumulative mortality recorded every 24 h after virus challenge. (B) Names and schematic representations of the full-length and truncated proteins used in this study. HsTM: transmembrane domain of human MAVS. (C and D) Interaction between CgMAVS and CgRIG-I-1 was detected by yeast two hybrid (Y2H) (C) and co-immunoprecipitation (Co-IP) (D) assays. DDO: -Leu/-Trp double-dropout media, QDO/X/A: -Ade/-His/-Leu/-Trp quadruple dropout media with X-α-Gal and aureobasidin. The transmembrane domain of CgMAVS is important in mediating this interaction.
