Figure 5

CgMAVS responds to poly(I:C) and OsHV-1 challenges and forms homodimers. (A and B) Expression profile of CgMAVS after poly(I:C) and OsHV-1 challenges analysed by qRT-PCR. (D and E) Dimerization of CgMAVS was analysed by Y2H (D) and Co-IP (E). DDO: -Leu/-Trp double-dropout media, QDO/X/A: -Ade/-His/-Leu/-Trp quadruple-dropout media with X-α-Gal and aureobasidin. The TM domain of CgMAVS was important in mediating this interaction.