Figure 6 | Scientific Reports

Figure 6

From: Characterization of the Mollusc RIG-I/MAVS Pathway Reveals an Archaic Antiviral Signalling Framework in Invertebrates

Figure 6

CgTRAF6 interacts with CgMAVS and specifically activates the NF-κB pathway. (A) Expression profile of CgTRAF6 after poly(I:C) challenge determined by qRT-PCR. (B and C) Interaction between CgTRAF6 and CgMAVS detected by Y2H (B) and Co-IP (C). DDO: -Leu/-Trp double-dropout media, QDO/X/A: -Ade/-His/-Leu/-Trp quadruple-dropout media with X-α-Gal and aureobasidin. (D) CgTRAF6 specifically activated the NF-κB promoter in a concentration-dependent manner. The activation was detected using dual luciferase reporter assays in human HEK293T cells.

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