Figure 3
From: Identification of novel microRNAs in the sheep heart and their regulation in heart failure

Analysis of microRNA microarray data from sheep left ventricular tissue. (A) Box plot showing the correlation R2 of 52 spike-ins for each sample with Cy3 and Cy5 labelling. Black dots indicate outliers. (B) MA-plot of a representative sample after Lowess (LOcally WEighted Scatterplot Smoothing) normalization. Green: Hy3 controls, red: spike-ins, black: all other probes. (C) Number of miRNA in each individual sample detected above background threshold (1.2 fold above 25th percentile). (D) The overlap of miRNAs detected by next generation deep sequencing (NGS) and miRNA array. (E and F) Correlation of array intensities with NGS counts and qPCR Cq values for 23 miRNAs. (Cont nโ=โ2, HF nโ=โ4, HF-R nโ=โ3).