Figure 7
ATF3 negatively regulates Atg5 gene. (a) Neuro2a cells were treated with either a control non-targeting siRNA or the Atf3-specific siRNA for 48 h and then infected with JEV (MOI 5). Relative expression of Atg5 at different times pi was studied by qRT PCR, and the mean is plotted (left panel). Cell lysates were prepared 24 h pi, and Western blotted for ATG5 and ATF3. Shown below is the ratio of ATG5/GAPDH. (b) MEF cells were treated with either a control non-targeting siRNA or the Atf3-specific siRNA for 48 h and then infected with JEV (MOI 5) (left panel) or transfected with 1 μg/ml poly(IC) (right panel). Relative expression of Atg5, 24 h post-treatment, was studied by qRT PCR, and the mean is plotted. Transcript level in control siRNA-treated cells is shown by the black bar while that in the Atf3 siRNA-treated cells is shown by the gray bar. The Gapdh levels were used for normalization. The experiments were done in triplicates on three different occasions. The statistical significance of the difference between the data was established by Student’s t-test; *p < 0.05; **p < 0.01; ***p < 0.001.
