Figure 8
ATF3 depletion leads to reduced JEV replication. (a) Neuro2a or MEF cells were treated with either a control non-targeting siRNA or the Atf3-specific siRNA for 48 h and then infected with JEV (MOI 5). Total RNA was isolated from the cells at 24 h pi and qRT PCR performed for quantitation of relative levels of JEV RNA. Gapdh levels were used for normalization. JEV RNA level in control siRNA-treated cells is shown by the black bar while that in the Atf3 siRNA-treated cells is shown by the gray bar. (c) Cellular supernatant collected from the above experiment was assayed for JEV titer. (d) Cell lysates prepared at 24 h pi from Neuro2a cells treated as above were Western blotted for JEV NS3 protein. The ratio of NS3/GAPDH is shown under the figure. The statistical significance of the difference between the data was established by Student’s t-test; *p < 0.05; **p < 0.01; ***p < 0.001.
