Figure 2
From: A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis
Stabilized β-catenin induces Osx expression in cementogenesis. (a) Immunohistochemical staining of the cervical cementum region of the distal root of the mandibular first molar in each group at P6W. Red arrows indicate strong Osx expression in cementoblasts and cementocyte-like cells in the excessive cementum in OC-Catnb mice. Scale bars, 20 μm. D, dentin; PDL, periodontal ligament; C, cementum. (b) The protein levels of β-catenin (β-Cat), Osx, and Runx2 were analyzed and compared by Western blotting. OCCM-30 cells were treated with OM for 1 day after transduction with retroviruses expressing two types of constitutively active mouse β-catenin, β-Cat S33Y, and β-Cat GSK. Samples shown are from the same experiment, and the gels/blots were processed under the same experimental conditions. β-Actin was used as a loading control. Cropped images are displayed here; the original full-size blots are presented in Supplementary Figure 2c. (c) Immunocytochemical staining was performed to look for colocalization of nuclear Osx and stabilized β-catenin. OCCM-30 cells were treated for 1 day with OM after transient transfection with plasmids driving the expression of β-Cat GSK. White arrowheads (β-catenin) and red arrowheads (Osx) indicate regions of colocalization of overexpressed stabilized β-catenin and nuclear Osx in the same cells. Nuclei were counterstained with DAPI. Scale bars, 50 μm.
