Figure 4
From: A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis
Stabilized β-catenin regulates cementogenesis in an Osx-dependent manner. (a) The transcript levels of various extracellular matrix genes including Bsp, Opn, OC, and Dmp1 were analyzed by real-time qPCR. RNA was isolated from control (shNC) and Osx-ablated (shOsx) OCCM-30 cells treated with OM for 4 days after retroviral transduction of control and β-Cat S33Y-expressing viruses. Each designated p-value indicates a significant difference between the relevant group and the negative control (control retrovirus + shNC). (b) After treatment with OM for 4 days after transduction with control or β-Cat S33Y-expressing retrovirus, ALP activities was analyzed and displayed with ALP staining. (c) Mineralization in OCCM-30 cells expressing shNC or shOsx was analyzed by Alizarin red staining and quantified under the same experimental conditions as above. Data are presented as mean ± SD of three measurements of each group. Significance was assigned for p-values as indicated.
