Figure 6
From: A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis
Osx regulates cementogenesis through Tcf/Lef. The transcript levels of various extracellular matrix genes including Bsp, Opn, OC, and Dmp1 were analyzed by real-time qPCR. RNA was isolated from (a) OCCM-30 cells and (b) Osx-ablated (shOsx) OCCM-30 cells treated with OM for 4 days after transient transfection of plasmids driving the expression of Gfp (as a control) and mouse Lef1. (c) Tcf/Lef binding activities were analyzed by the FOPflash/TOPflash luciferase reporter assay. OCCM-30 cells expressing shOsx or shNC were transiently transfected with gradually increasing concentrations of a plasmid driving the expression of Lef1 and then treated with OM for 1 day. (d) The transcript levels of various genes in the Tcf family, including Lef1, Tcf1, Tcf3 and Tcf4, were analyzed by real-time qPCR. RNA was isolated from OCCM-30 cells expressing shOsx or shNC after treatment with OM for 1 day. All data are presented as mean ± SD of three measurements in each group. Significance was assigned for p-values as indicated.
