Figure 6
From: Rap1 and Cdc13 have complementary roles in preventing exonucleolytic degradation of telomere 5′ ends
The Rap1 DBD is sufficient and the wrapping loop is essential for mediating 5′ end protection. (a) DEPA on D17S9 was performed after pre-incubation with BSA (0.8 μg), Rap1 DBD337–582 (0.8 μg), DBD337–572 (0.4 μg) or DBD337–582 (0.8 μg), for 0; 20; 40; 60; 120; 240 s. The nucleotide sequence representing each band and its position relative to the Rap1 MBS is shown to the right. The arrow points out the position of the stalling product. LC indicates the loading control. Lane number is below the gel. (b) The results shown in (a) were quantified and the % of uncleaved full length substrate at each time point was calculated.
