Figure 3 | Scientific Reports

Figure 3

From: Glaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin

Figure 3

Rat retinal lysates from control and high IOP rats were subjected to western blotting and probed for (A) neuroserpin and (B) plasmin immunoreactivity. Actin was used as loading control. Blots were cropped to show the relevant band. The relative intensities of WB bands were quantified in both cases and plotted (n = 5 each). (C) Double immunostaining of the rat retinal sections from control and microbead induced high IOP eyes illustrating neuroserpin (red) and plasminogen (green) expression and localization in various retinal layers (Blue-DAPI). (n = 5; scale 50 µm). (D) Relative fluorescence intensities of neuroserpin and plasmin immunoreactivity in sections were quantified using ImageJ programme in GCL and INL and plotted.

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