Figure 6
Neuroserpin was immunoprecipitated from (A) control and glaucoma human and (B) control and high IOP rat retinas. The eluted immunoprecipitates were subjected to western blotting and probed using plasmin and neuroserpin antibodies. Human retinal lysates alone were also loaded as controls. Plasmin was immunoprecipitated from (C) human and (D) rat retinal lysates from control and glaucoma samples along with corresponding IgG control immunoprecipitations. Human and rat retinal lysates respectively were loaded in C and D. After elution, the immunoprecipitates were subjected to western blotting and probed using plasmin and neuroserpin antibodies. High molecular weight bands (indicated by arrow) implicating neuroserpin-plasmin complex formation were observed in both human and rat glaucomatous retinal samples. Blots were cropped to show the relevant bands.
