Figure 7
Neuroserpin was immunoprecipitated from control and glaucomatous (A) human retinas (B) vitreous and from (C) the rat retinas. The immunoprecipitates were blotted and probed for methionine sulfoxide immunoreactivity. The blots were also probed with anti-neuroserpin antibody in each case. Blots were cropped to show the relevant bands. Relative band intensities were quantified in each case and plotted (n = 4; *p < 0.001). (D) Rat retinal sections from control and high IOP induced eyes were immunostained with either IgG control or met sulfoxide antibody (red), DAPI (blue). Arrows (white) highlight the increased met sulfoxide staining in the inner retinal layers of high IOP subjected rats. The average relative fluorescence intensities for met sulfoxide reactivity in sections (n = 4) were quantified in GCL and INL of retinas using ImageJ, compared using students t-test and plotted with standard deviation and error bars (Scale 50 µm, *p < 0.04).
