Figure 2

Effects of DES on serum gonadotropin levels and expression of ovarian cognate receptors. Concentrations of LH (A) and FSH (C) in immature female rats treated with DES for the indicated time. Data for each point are from five animals (the means ± SEM). Expression of ovarian LH receptor (LHR; B) and FSH receptor (FSHR; D) in immature rats treated with DES for the indicated time. Expression of each gene was analyzed by Q-PCR and normalized to 36B4 expression. Q-PCR data represent the mean ± SEM of at least four independent samples. Values marked by different letters are significantly different (P < 0.05).