Figure 5

Effects of DES on the ovarian steroidogenesis-related proteins and the concentrations of serum steroid hormones. (A) Localization of Star, Cyp11a1, 3β-Hsd and Cyp17a1 proteins in immature ovaries before or 6 h after DES injection. Positive staining both for each protein was observed in theca cells. (B–D) Serum progesterone, testosterone and E2 levels in immature female rats treated with DES for indicated times. Data represent the mean ± SEM of at least 5 independent samples. Values marked by different letters are significantly different (P < 0.05).