Figure 4

RNAi response in A. glabripennis larvae. (A) IAP mRNA levels on 5th day after dsIAP injection. Total RNA isolated from A. glabripennis larvae injected with dsGFP or dsIAP was used in RT-qPCR to quantify IAP mRNA levels using GAPDH mRNA levels for normalization. The asterisk indicates significant differences in mRNA levels (t-test, one-tailed, P-value 0.001, N = 4). (B) Survival rate (%) on 10th day after injection of dsRNA. The asterisk indicates significant differences in mortality between treatment and control (Fisher’s Exact test, Two-tailed, P-value < 0.001, N = 10).