Table 1 RNAi genes identified in the genome of A. glabripennis and their predicted biological function.
Group | Genes | ALB Accession | Accession # | Identity | Biological function | Reference |
|---|---|---|---|---|---|---|
Core RNAi machinery | Dicer 1 | AGLA015685-PA | TC001750 | 71% | RNase III, conversion of pre-miRNA to miRNA | |
Dicer 2 | AGLA013917-PA | TC001108 | 55% | RNase III, processing of long dsRNA into siRNAs | ||
Ago1 | AGLA017309-PA | TC005857 | 99% | Catalytic subunit of RISC | ||
Ago2 | AGLA012768-PA | TC011525 | 60% | |||
Ago3 | AGLA010388-PA | TC008511 | 56% | |||
Piwi/Aub | AGLA006316-PA | TC008711 | 64% | |||
Drosha | AGLA019222-PA | TC016208 | 92% | RNase III, cleavage of pri-miRNA to pre-miRNA | ||
R2D2 | AGLA013618-PA | TC008716 | 44% | dsRNA binding co-factor of Dicer-2 | ||
Pasha | AGLA000778-PA | TC015332 | 69% | miRNA biogenesis | ||
Loquacious | AGLA003298-PA | TC01166 | 56% | dsRNA binding co-factor of Dicer-1 | ||
Vesicle mediated transport | Arf72A | AGLA012021-PA | TC008443 | 98% | Endosome transport | |
AP50 | AGLA021602-PA | TC011923 | 99% | Endocytosis | ||
Clathrinhc | AGLA019383-PA | TC015014 | 97% | Endocytosis | ||
Rab7 | AGLA012748-PA | TC006036 | 76% | Endosome transport | ||
Proton transport | Vha16 | AGLA014759-PA | TC011025 | 92% | ATP synthase/ATPase | |
VhaSFD | AGLA018530-PA | TC006281 | 85% | ATP synthase/ATPase | ||
Intracellular transport | IdICP | AGLA000215-PA | TC010886 | 60% | Exocytosis | |
Light | AGLA019229-PA | TC015204 | 85% | Lysosomal transport | ||
NinaC | AGLA004194-PA | TC014087 | 37% | Rhodopsin mediated signaling | ||
Lipid metabolism | Gmer | AGLA010431-PA | TC006281 | 78% | Metabolism | |
P13K59F | AGLA002972-PA | TC000620 | 79% | Lipid metabolism | ||
Saposinr | AGLA006229-PA | TC000449 | 62% | Lipid metabolism | ||
dsRNA uptake proteins | SilA | AGLA015910-PA | TC011760 | 52.4% | dsRNA transporter | |
SilC | AGLA005113-PA | TC015033 | 59.9% | |||
Miscellaneous proteins | Egghead | AGLA003739-PA | TC008154 | 84% | Oogenesis | |
Eater | AGLA015417-PA | TC030481 | 38% | Innate immune response/phagocytosis | ||
Scavenger receptor | AGLA006342-PA | TC015640 | 53% | Endocytosis | ||
RNA helicase DDX | AGLA008916-PA | TC010546 | 50% | DEAD-box RNA helicase, required for RNAi | ||
Epsin-like (rsd-3) | AGLA017225-PA | TC012168 | 64% | Systemic RNAi | ||
Liquid facets (Epn-1) | AGLA021257-PA | TC005393 | 97% | Endocytic protein | ||
dsRNase enzymes | dsRNase1 | XP_018564211 | XP_015840884 | 45% | Extra cellular endonuclease | |
dsRNase2 | XP_018561279 | AEE63490.1 | 48% | |||
dsRNase3 | XP_018579804 | XP_970494.1 | 41% |
