Figure 2 | Scientific Reports

Figure 2

From: Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan

Figure 2

The Fourier domain model of the oblique scanning laser microscopy (OSLM). (A) The range of the incidence and detection in the 3D reciprocal Fourier domain. The blue area on the spherical dome represents the illumination, and the overlapping area projected onto the dome from two collection cones of OL2 and OL4 shows the detectable spatial frequency range by OSLM. (B) The incident, (C) emission and (D) combined point spread functions (PSFs) of the system. (E) The theoretical resolutions in three directions under various numerical aperture of the objective lens, under the same detection range in (A). The resolution is measured by FWHM crossing the center of the 3D PSF in panel D.

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