Figure 1 | Scientific Reports

Figure 1

From: Integrated roles of BclA and DD-carboxypeptidase 1 in Bradyrhizobium differentiation within NCR-producing and NCR-lacking root nodules

Figure 1

The USDA110 bclA gene confers sensitivity to the antibiotic bleomycin and the antimicrobial peptide Bac7. (a) Bleomycin sensitivity in B. diazoefficiens strain USDA110 and its bclA mutant derivative and in E. coli strain BW25113ΔsbmA and S. meliloti strain Sm1021ΔbacA carrying an empty vector or a vector expressing USDA110 bclA. Blue bars are for strains expressing bclA and red bars are for strains lacking the gene. Bleomycin concentrations were applied as indicated and growth was determined by optical density measurement at 600 nm with a plate reader after 72 h, 48 h or 24 h incubation for USDA110, Sm1021 and BW25113 respectively. (b) Survival of E. coli strain BW25113ΔsbmA (top) and S. meliloti strain Sm1021ΔbacA (bottom) derivatives carrying the empty pRF771 plasmid or the USDA110 bclA gene located on plasmid pRF771 after treatment with the peptide Bac7 at the indicated concentration. The surviving bacteria were counted and expressed as % from the water control treatment. Error bars in all panels are standard deviations.

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