Figure 3 | Scientific Reports

Figure 3

From: Integrated roles of BclA and DD-carboxypeptidase 1 in Bradyrhizobium differentiation within NCR-producing and NCR-lacking root nodules

Figure 3

The bclA gene of B. diazoefficiens USDA110 complements the bclA mutation in Bradyrhizobium sp. ORS285ΔbclA and the bacA mutation in S. meliloti Sm1021ΔbacA. (a,c,e) Phenotype of A. indica nodules at 14 dpi, infected with the indicated strains. Scale bars are 1 mm. (b,d,f) Bacteroid viability determined by live/dead and calcofluor white staining of nodule sections and confocal microscopy in A. indica nodules induced by the indicated strains. Scale bars are 10 µm. (g,i,k) Phenotype of M. sativa nodules at 28 dpi, infected with the indicated strains. Scale bars are 1 mm. (h,j,l) Bacteroid viability determined by live/dead staining of nodule sections and confocal microscopy in M. sativa nodules induced by the indicated strains. Scale bars are 10 µm.

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