Figure 6 | Scientific Reports

Figure 6

From: Integrated roles of BclA and DD-carboxypeptidase 1 in Bradyrhizobium differentiation within NCR-producing and NCR-lacking root nodules

Figure 6

The Bradyrhizobium strain ORS285 ΔbclA, DD-CPase1, ΔbclA/DD-CPase1 mutant phenotypes in symbiosis with A. afraspera and A. indica. (a) Confocal microscopy of bacteroid viability determination by live/dead staining of A. afraspera and A. indica nodules induced by ORS285 wild type and the indicated mutants. Scale bars are 10 µm. (b) Relative nitrogen fixation activity (% of maximum/h/plant) of A. afraspera and A. indica plants inoculated with ORS285 wild type and the indicted mutants, measured by the acetylene reduction assay per plant. Box-plots represent in the rectangle the first quartile to the third quartile, divided by the median value, whiskers above and below the box show the minimum and maximum measured values.

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