Figure 4 | Scientific Reports

Figure 4

From: The function of the inner nuclear envelope protein SUN1 in mRNA export is regulated by phosphorylation

Figure 4

The LINC complex in mRNA export. (A) Efficiency of Nesprin-2 knockdown (KD) by shRNA treatment in HeLa cells is shown by immunofluorescence with Nesprin-2 specific antibodies pAbK1 and by western blot analysis. The quantitative determination of the knockdown is depicted in a bar graph (left). Scale bar, 5 µm. (B) RNA FISH analysis of Nesprin-2 depleted cells as shown by immunofluorescence analysis. Left side, distribution of the N/C intensity range. (C) The mean nuclear/cytoplasmic (N/C) intensity ratio of control and Nesprin-2 KD cells. The values represent the mean ± SD of more than 100 cells analyzed. (D) Immunofluorescence analysis of GFP-SUN1-ΔSUN expressing cells. Nesprin-2 was detected by pAbK1. Scale bar, 10 µm.

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