Figure 6

HCMV downregulation of B7-H6 leads to evasion from NKp30-mediated killing by NK cells. (A) NK cell killing of ARPE19 cells infected with different HCMV strains as depicted in the figure legend. Two effector to target ratios are presented as depicted on the X-axis. One representative experiment out of four is presented. (B) NK cell killing of ARPE19 cells infected with different HCMV strains and blocked using different antibodies as depicted in the figure legend. The isotype control antibody used was an anti-human CD99 antibody. Blocking experiments were performed at a 20:1 effector to target ratio. One representative experiment out of four is presented. (C) NK cell killing of ARPE19 cells infected with different HCMV strains and blocked with either isotype control antibody (black bars, polyclonal mouse IgG1) or anti-B7-H6 antibody (grey bars). One representative experiment out of two is presented. For all presented experiments error bars represent standard error of the mean. A student’s t test was performed in order to evaluate significance. *p-value < 0.05. **p-value < 0.005. N.S. = non-significant differences.