Figure 1
From: Cardiac injury of the newborn mammalian heart accelerates cardiomyocyte terminal differentiation
Effect of AR on cardiomyocyte centrosome disassembly. (a) Representative images of centrioles (γ-tubulin) in heart cryosections of P0 rat heart ventricles. Nuclei: DAPI. Cardiac nuclei: Nkx2.5. Arrowheads indicate paired centrioles in non-myocytes. Asterisk indicates centrioles in cardiomyocytes. Paired-centrioles: doublet γ-tubulin signals within 2 μm of one another; split-centrioles: γ-tubulin signals greater than 2 μm of one another; single-centriole: single γ-tubulin signal with no identifiable pair (e.g. other centriole is either overlapping or split to the extent of no longer residing in the section); no-centriole: no identifiable, nuclear-proximal, γ-tubulin signal in the section. Scale bars: 2 μm. (b,c) Quantitative analysis of centriole signals and configurations in non-myocytes (b) and cardiomyocytes (c) from cryosections of P0 (MOCK) and P3 or P6 (SHAM and AR) rat heart ventricles. Results are from three independent animals. ≥200 cardiomyocytes and ≥100 non-myocytes, collectively, from basal and apical regions were analyzed per experimental condition (see also Supplementary Fig. 1). (d) Quantitative analysis of cardiomyocytes proximal (within 1 mm) to base or apex/resection with paired centrioles from cryosections of P3 or P6 (SHAM and AR) rat hearts. Data are ± SD. p-values were calculated using two-tailed Student’s t-test.
