Figure 2
From: Cardiac injury of the newborn mammalian heart accelerates cardiomyocyte terminal differentiation
Analysis of cardiomyocyte binucleation in AR hearts. (a) Representative images of mononucleated and binucleated P8-isolated rat cardiomyocytes. Nuclei were counterstained with propidium iodide. Yellow scale bars: 10 μm. (b,c) Percent binucleated cardiomyocytes at different time points after AR and SHAM in P1 rat (n = 4 for each time point, (b) and mouse (n = 3 for each time point, (c), see also Supplementary Fig. 2) hearts. p-values were calculated using two-tailed Student’s t-test comparing AR and SHAM. Inflection points reflect mean values. ΔR = fold difference in the rate of binucleation of AR- compared to SHAM-operated hearts. P1 data point for rat is from Li et al. (JMCC, 1996) and for mouse from Soonpaa et al. (AJP, 1996). p-values considered here significant (p < 0.05) are indicated in red.
