Figure 3

Analysis of H3P-positive cardiomyocytes in AR rat hearts. (a) Representative immunofluorescence images of a P6 H3P-positive cardiomyocyte and non-myocyte (scale bar: 50 µm). Red: cardiomyocytes (sarcomeric-α-actinin); green: cells in mitosis (H3P); blue: nuclei (DAPI). Scale bar: 50 µm (see also Supplementary Fig. 3). (b) Quantification of cardiomyocytes in mitosis at P3 and P6 based on anti-H3P and anti-sarcomeric-α-actinin staining. Nuclei were counterstained with DAPI. Data are ± SD. n = 4 for MOCK, AR, and SHAM at each time point. (c,d) Quantification of H3P-positive cardiomyocytes at P3 (c) and P6 (d) in apical, mid, and base zones. Data are ± SD. n = 4 for MOCK, AR, and SHAM at each time point. p-values have been calculated using Fisher’s LSD test after one-way Anova.