Figure 5
From: Cardiac injury of the newborn mammalian heart accelerates cardiomyocyte terminal differentiation
Characterization of AR rat hearts. (a) Representative stereomicroscopic images of SHAM- and AR-operated rat hearts at different postnatal time points. Arrow heads indicate myocardial scar. Scale bars: 1 mm. (b–d) Representative images of Hematoxylin & Eosin staining (b), Masson’s trichrome staining (c), and immunofluorescence analysis of non-myocytes (Collagen I/Desmin/DAPI) (d) of SHAM- and AR-operated hearts at different postnatal time points. P3–P22 (n = 4–6) and P56 (n = 3) for each group. Red scale bars: 1 mm. Black/yellow scale bars: 200 µm. (e) The mean wall motion of each heart segment was compared between SHAM and AR hearts (n = 3). (f) Summary of wall motion depicting heart segments in AR hearts versus those of SHAM demonstrates defects in the apex region. (g) Quantitation of heart axes by PET scanning (n = 3). Right-left (RL). Anterior-posterior (AP). Data are ± SEM. *p < 0.05.
