Figure 3

Western blot analysis of ubiquitylated proteins associated with the polyhedra matrix of BmNPV. Polyhedra matrix proteins were separated on SDS-PAGE gel and then analyzed using mouse monoclonal anti-ubiquitin antibody clone FK2 (A) and FK1 (B), rabbit monoclonal anti-ubiquitin antibody Lys48-specific clone Apu2 (C) and Lys63-specific clone Apu3 (D), and mouse monoclonal anti-linear poly-ubiquitin antibody clone LUB9 (E). Images were visualized using a secondary antibody conjugated to HRP and a chemiluminescence detection system.