Figure 6

BM134 as a carrier to incorporate eGFP into the polyhedra matrix. (A) PCR identification of the ORF134 knockout bacmid. Lane M, DNA marker. Lane 1, DNA template of wildtype BmNPV bacmid and lane 2, template of the ORF134-disrupted bacmid, vBM134^KO. (B) Construction of the BM134-eGFP-encoded nucleotide sequence- and polyhedrin-inserted bacmids vBM134^Re-eGFP and vBM134^KO-Polh. (C) Fluorescence of the polyhedra purified from BmN cells co-infected with viral stocks of vBM134^Re-eGFP and vBM134^KO-Polh. (D) Western blot analysis of purified polyhedra immobilized with the fusion protein BM134-eGFP using a mouse monoclonal anti-eGFP antibody. Lane M, pre-stained protein marker.