Table 1 Host proteins identified in the BmNPV polyhedral matrix using the LC-ESI-MS/MS Q Exactive technique.

From: Protein composition analysis of polyhedra matrix of Bombyx mori nucleopolyhedrovirus (BmNPV) showed powerful capacity of polyhedra to encapsulate foreign proteins

Classification

Protein

Pfam domain(s)

Abbreviation

Size (amino acids)

Gel region(s) (No. of peptides identified; Coverage (%))

Chaperones

BGIBMGA002381-PA

70 kilodalton heat shock cognate/protein

HSP70-4

649

M1 (5; 10.63), M2 (16; 34.98), M3 (8; 18.03)

BGIBMGA006196-PA

40 kilodalton heat shock protein

HSP40

313

M3 (3; 13.74)

BGIBMGA002429-PA

Cyclophilin type peptidyl-prolyl cis-trans isomerase/cyclophilin-like domain

Cyclophilin A

165

M5 (6; 58.18)

BGIBMGA004331-PA

FK506 binding protein

FKBP

108

M5 (2; 32.41)

Ubiquitin and related proteins

BGIBMGA011805-PA

Ubiquitin carboxyl-terminal hydrolase

UCH

665

M1 (1; 4.36), M2 (1; 2.56)

BGIBMGA011581-PA

Small ubiquitin-related modifier

SUMO

91

M1 (1; 13.19), M2 (1; 13.19), M3 (1; 13.19)

BGIBMGA001549-PA

Ubiquitin

Ubiquitin

153

M3 (3; 26.80), M5 (3; 26.80)

Helicases

BGIBMGA011746-PA

DEAD/DEAH box helicase

DEAD/H

569

M1 (3; 7.56), M2 (6; 16.52), M3 (11; 26.89)

BGIBMGA011965-PA

DEAD/DEAH box helicase

DEAD/H

628

M2 (5; 13.69), M3 (8; 20.70)

BGIBMGA010673-PA

DEAD/DEAH box helicase

DEAD/H

372

M3 (9; 33.60)

BGIBMGA004822-PA

DEAD/DEAH box helicase

DEAD/H

405

M3 (4; 15.31)

Cytoskeleton- related proteins

BGIBMGA005576-PA

Actin

Actin

344

M1 (4; 23.84)

BGIBMGA002981-PA

Profilin

Profilin

126

M5 (3; 47.62)

BGIBMGA007092-PA

Calponin homology domain, calponin family repeat

CH

173

M5 (6; 54.91)

RNA binding proteins

BGIBMGA006405-PA

RNA recognition motif

RRM

361

M3 (5; 15.80)

BGIBMGA014155-PA

K Homology domain

KH

377

M3 (6; 27.98)

BGIBMGA004881-PA

RNA recognition motif

RRM

252

M3 (4; 23.02), M5 (3; 17.86)

BGIBMGA013464-PA

RNA recognition motif

RRM

287

M3 (3; 15.33)

BGIBMGA007410-PA

RNA recognition motif

RRM

314

M3 (3; 15.61)

BGIBMGA013100-PA

Like Sm protein

LSm

130

M5 (2; 23.85)

Others

BGIBMGA003126-PA

Nup93/Nic96

NIC

820

M1 (3; 5.24),

BGIBMGA000808-PA

XRN 5’-3′ exonuclease

XRN_N

322

M1 (2; 8.39),

BGIBMGA005650-PA

CAF1 family ribonuclease

 

479

M1 (3; 8.77), M2 (3; 8.77),

BGIBMGA008447-PA

Nuclear transport factor 2 domain, Nuclear RNA export factor 1

NTF2 NXF1

613

M2 (2; 4.89),

BGIBMGA001106-PA

Ribosomal protein L21e

 

159

M5 (3; 24.53)

BGIBMGA006867-PA

Ribosomal protein S8

 

129

M5 (2; 23.26)

BGIBMGA011282-PA

Ribosomal S13/S15 N-terminal domain

 

151

M5 (2; 19.21)

BGIBMGA009106-PA

glutathione S-transferase 2

GST-2

416

M5 (4; 13.22)