Table 1 Host proteins identified in the BmNPV polyhedral matrix using the LC-ESI-MS/MS Q Exactive technique.
Classification | Protein | Pfam domain(s) | Abbreviation | Size (amino acids) | Gel region(s) (No. of peptides identified; Coverage (%)) |
---|---|---|---|---|---|
Chaperones | BGIBMGA002381-PA | 70 kilodalton heat shock cognate/protein | HSP70-4 | 649 | M1 (5; 10.63), M2 (16; 34.98), M3 (8; 18.03) |
BGIBMGA006196-PA | 40 kilodalton heat shock protein | HSP40 | 313 | M3 (3; 13.74) | |
BGIBMGA002429-PA | Cyclophilin type peptidyl-prolyl cis-trans isomerase/cyclophilin-like domain | Cyclophilin A | 165 | M5 (6; 58.18) | |
BGIBMGA004331-PA | FK506 binding protein | FKBP | 108 | M5 (2; 32.41) | |
Ubiquitin and related proteins | BGIBMGA011805-PA | Ubiquitin carboxyl-terminal hydrolase | UCH | 665 | M1 (1; 4.36), M2 (1; 2.56) |
BGIBMGA011581-PA | Small ubiquitin-related modifier | SUMO | 91 | M1 (1; 13.19), M2 (1; 13.19), M3 (1; 13.19) | |
BGIBMGA001549-PA | Ubiquitin | Ubiquitin | 153 | M3 (3; 26.80), M5 (3; 26.80) | |
Helicases | BGIBMGA011746-PA | DEAD/DEAH box helicase | DEAD/H | 569 | M1 (3; 7.56), M2 (6; 16.52), M3 (11; 26.89) |
BGIBMGA011965-PA | DEAD/DEAH box helicase | DEAD/H | 628 | M2 (5; 13.69), M3 (8; 20.70) | |
BGIBMGA010673-PA | DEAD/DEAH box helicase | DEAD/H | 372 | M3 (9; 33.60) | |
BGIBMGA004822-PA | DEAD/DEAH box helicase | DEAD/H | 405 | M3 (4; 15.31) | |
Cytoskeleton- related proteins | BGIBMGA005576-PA | Actin | Actin | 344 | M1 (4; 23.84) |
BGIBMGA002981-PA | Profilin | Profilin | 126 | M5 (3; 47.62) | |
BGIBMGA007092-PA | Calponin homology domain, calponin family repeat | CH | 173 | M5 (6; 54.91) | |
RNA binding proteins | BGIBMGA006405-PA | RNA recognition motif | RRM | 361 | M3 (5; 15.80) |
BGIBMGA014155-PA | K Homology domain | KH | 377 | M3 (6; 27.98) | |
BGIBMGA004881-PA | RNA recognition motif | RRM | 252 | M3 (4; 23.02), M5 (3; 17.86) | |
BGIBMGA013464-PA | RNA recognition motif | RRM | 287 | M3 (3; 15.33) | |
BGIBMGA007410-PA | RNA recognition motif | RRM | 314 | M3 (3; 15.61) | |
BGIBMGA013100-PA | Like Sm protein | LSm | 130 | M5 (2; 23.85) | |
Others | BGIBMGA003126-PA | Nup93/Nic96 | NIC | 820 | M1 (3; 5.24), |
BGIBMGA000808-PA | XRN 5’-3′ exonuclease | XRN_N | 322 | M1 (2; 8.39), | |
BGIBMGA005650-PA | CAF1 family ribonuclease | Â | 479 | M1 (3; 8.77), M2 (3; 8.77), | |
BGIBMGA008447-PA | Nuclear transport factor 2 domain, Nuclear RNA export factor 1 | NTF2 NXF1 | 613 | M2 (2; 4.89), | |
BGIBMGA001106-PA | Ribosomal protein L21e | Â | 159 | M5 (3; 24.53) | |
BGIBMGA006867-PA | Ribosomal protein S8 | Â | 129 | M5 (2; 23.26) | |
BGIBMGA011282-PA | Ribosomal S13/S15 N-terminal domain | Â | 151 | M5 (2; 19.21) | |
BGIBMGA009106-PA | glutathione S-transferase 2 | GST-2 | 416 | M5 (4; 13.22) |