Table 2 Viral proteins identified in the BmNPV polyhedral matrix by a Q Exactive LC-ESI-MS/MS analysis.

From: Protein composition analysis of polyhedra matrix of Bombyx mori nucleopolyhedrovirus (BmNPV) showed powerful capacity of polyhedra to encapsulate foreign proteins

BmNPV ORF

AcMNPV ORF

Protein designation

Predicted molecular mass (kDa)

Location in ODV virion†

Location in BV virion†

OB formation when deleted? (ODV embedded?)

Gel region(s) (No. of peptides identified; Coverage (%))

1

8

Polyhedrin

28.8

ENC17

NC17

No

M1 (18; 64.9), M2 (28; 97.55), M3 (27; 97.55), M4 (25; 97.55), M5 (23; 96.33)

2

9

P78/83

60.9

NC38, 39, ENC17

NC17, 38, 39

 

M2(6; 16.42), M3 (3; 9.23), M5 (1; 2.40)

3

10

PK1

32.4

NC40

NC40

No41

M3 (1; 4.36)

4

11

 

39.8

  

Yes(No)42

M1 (1; 4.12), M3 (9; 36.47)

5

13

 

39.3

  

Yes(Yes)43

M4 (1; 2.42)

7

15

EGT

57.0

  

Yes(Yes)44

M5 (1; 2.77)

8

16

BV/ODV-E26

26.2

ENC45

ENC45

Yes(Yes)44

M5 (1; 6.11)

9

17

 

24.1

 

ENC17

Yes(Yes)44, 46

M5(2; 14.29)

10

18

 

41.5

  

Yes(Yes)47

M1 (1; 5.06), M3 (6; 24.44)

11

19

 

12.5

   

M2 (1; 15.45)

14

23

F protein

78.0

E12

E17, 22

Yes(Yes)48, 49

M1 (1; 2.23)

15

24

PKIP

19.4

 

ENC17

No50

M5 (7; 49.70)

17

26

 

14.5

Unknown17

  

M1 (1; 9.30), M5 (7; 25.58)

19

28

LEF-6

20.3

 

Unkown17

 

M5 (1; 6.36)

22a

      

M3 (1; 20.37)

23

31

SOD

16.3

E17

 

Yes(Yes)51

M1 (2; 19.87), M3 (2; 15.23), M5(5; 53.64)

26

35

Ubiquitin

8.7

E17, 52

E17, 22, 52

Yes35

M1 (2; 32.47), M2 (1; 20.78), M3 (3; 49.35), M5(2; 44.16)

27

36

PP31

31.5

 

Unknown22

Yes(Yes)53

M1 (1; 5.78), M2 (1; 5.42)

29

38

 

25.5

 

E54

 

M1 (1; 3.69), M3 (1; 4.15), M5 (1; 5.07)

30

39

P43

43.3

  

Yes55

M2 (1; 4.14), M3 (1; 4.14)

34

43

 

9.0

  

Yes, but few56, 57

M3 (1; 19.23), M5(6; 61.54)

36

45

 

22.5

   

M3 (1; 5.18)

37

46

ODV-E66

79.2

E12, 17, 22, 58

 

Yes(Yes)59

M1(10; 21.08), M2(21; 49.29), M3(17; 41.60), M5(5; 13.25)

38

47

ETS/TRAX

10.5

   

M5 (1; 12.36)

40

51

 

37.8

ENC17

Unknown22, 60

 

M3 (3; 13.48)

43

54

VP1054

42.0

NC17

NC61

Yes(No)62, 63

M3 (2; 7.12)

47

58/59

ChaB

37.8

NC17, 64

NC17

 

M5(4; 46.2)

48

60

 

9.7

   

M1 (1; 12.05), M3 (1; 12.05), M5(2; 13.25)

49

61

FP25K

25.3

NC65, ENC17

NC17, 65

Yes, but few(Yes, but few)66,67,68

M5 (1; 8.88)

51

63

 

18.5

 

E69

 

M4 (1; 5.81)

53

65

DNAPOL

114.4

Unknown17

 

No70

M1 (1; 2.64)

54

66

 

93.3

ENC17, E71

NC17

Yes72

M1(8; 15.16), M2(2; 3.85)

55

67

LEF-3

44.9

Unknown16

  

M2(1; 3.90), M3(6; 20.26), M4(1; 3.38), M5 (1; 2.60)

57

69

 

30.4

  

Yes(Yes)73

M5 (1; 7.63)

59

73

 

11.5

 

Unknown22

Yes(Yes)74

M5(4; 58.59)

60

74

 

31.0

Unknown12

E17

 

M3 (1; 3.36)

61

75

 

15.5

E17

ENC17

 

M5 (5; 35.34)

62

76

 

9.6

E75

E75

Yes(No)76

M1 (1; 15.29), M5(2; 16.47)

63

77

VLF-1

44.3

ENC17

NC77

Yes, but few (No)62, 78

M1 (6; 17.41), M2 (3; 9.50), M3 (8; 26.65), M5(4; 12.93)

65

79

 

12.2

Unknown12

 

Yes(Yes)79

M5 (2; 17.31)

66

80

GP41

44.9

ENC17

ENC17

Yes62

M1 (12; 41.69), M2 (6; 17.12), M3 (15; 59.31), M4(1; 6.20), M5(2; 9.18)

67

81

 

27.0

Unknown17

 

Yes(No)80

M2 (1; 4.27), M5 (1; 5.56)

68

82

TLP

20.1

 

E81

Yes(Yes)81

M2 (2; 25.41), M4(1; 4.42)

69

83

 

95.8

E17, ENC82

 

Yes(No)83, 84

M1 (2; 4.53), M2 (2; 4.77), M5 (1; 2.26)

70

87

VP15

15.1

   

M1 (1; 17.46), M2 (1; 17.46), M3 (1; 17.46)

71

88

CG30

30.7

Unknown12

 

Yes(Yes)85, 86

M1 (1; 5.24), M3 (1; 6.37)

72

89

VP39

39.3

NC17, 22, 87

NC17, 22, 87

 

M1 (1; 4.00), M3 (4; 20.29), M5 (1; 4.00)

75

92

P33

30.9

ENC17, 88

ENC89

Yes(No and singly enveloped nucleocapsid)88

M1(7; 22.01), M2(7; 32.05), M3(8; 28.96), M4(16; 77.22), M5(14; 62.93)

76

93

  

NC90

ENC90

Yes(No)90

M5(4; 32.92)

77

94

ODV-E25

25.6

E17, 22, 91

E17, 22

Yes(No)92

M1 (2; 15.35), M2 (1; 7.89), M3 (3; 28.07), M5(10; 60.96)

78

95

DNA helicase/P143

143.6

Unknown12, 17, 22

  

M2 (1; 0.65), M5 (1; 1.15)

79

96

PIF-4

21.0

E17, 93,94,95

E95

Yes95

M5 (1; 8.79)

80

 

BRO-b

27.5

   

M2 (1; 4.18)

81

 

BRO-c

35.9

   

M4(1; 2.83), M5 (2; 7.55)

82

98

38 K

38.0

NC17, 96

NC17, 96

Yes(No)97

M3 (3; 11.88)

84

100

P6.9

8.1

NC17, 98

NC17, 98

Yes(No)99

M1 (1; 12.31)

85

101

BV/ODV-C42

41.6

NC17, 100

NC17, 101

 

M3 (5; 21.55), M5 (1; 1.93)

87

103

P48

45.4

 

ENC17

Yes(No)102

M1 (5; 12.66), M2 (3; 10.34), M3 (12; 39.02), M5 (2; 5.17)

88

104

VP80

79.9

ENC17, NC103, 104

NC103, 104

 

M1 (9; 19.65), M2 (10; 23.12), M3 (7; 15.75)

91

108

 

11.8

E105, 106

 

Yes(Yes)106

M3 (1; 27.62)

92

109

ODV-EC43

45.0

ENC17, 107

ENC108

Yes(No)108,109,110

M1 (5; 18.41), M3 (14; 53.96), M5(4; 12.79)

92a

  

6.8

   

M2 (1; 47.46)

93

111

 

8.2

   

M1 (1; 20.09), M5 (1; 14.93)

94

114

 

49.4

Unknown12, 14, 111

Unknown22

Yes(Yes)112

M1 (3; 10.14), M3 (11; 39.86), M5(3; 12.74)

95

115

PIF-3

23.0

E17, 113

 

Yes(Yes)114, 115

 

95a

116

     

M1 (1; 25.00)

98

120

 

9.5

   

M5 (1; 13.41)

100

123

PK2

26.0

   

M5 (6; 28.89)

104

127

V-cath

36.9

 

ENC116

 

M1 (1; 3.10), M5 (4; 17.96)

106

129

P24

21.8

NC17, 117

NC17, 117

 

M1 (6; 37.44), M2 (5; 46.15), M3 (8; 58.97), M4 (2; 11.28), M5 (15; 73.33)

107

130

GP16

12.1

  

Yes(Yes)118

M5 (1; 10.38)

108

131

PEP

35.4

NC17

NC17

Yes(Yes)119

M1 (5; 16.51), M2 (2; 9.52), M3 (7; 32.70), M4 (3; 19.68), M5(5; 26.03)

109

132

 

25.2

NC120

NC120

Yes(Few)120

M4 (1; 4.09)

110

133

ALK-EXO

48.5

Unknown12

  

M3 (1; 1.67)

110a

      

M3 (1; 28.07)

111

     

Yes(Yes)121

M3 (1; 20.00)

112

135

P35

34.5

  

Yes(Yes)53, 122

M3 (6; 24.08), M5(2; 11.04)

114

137

P10

7.5

  

Yes(Yes)119, 123,124,125

M5 (1; 27.14)

115

138

P74/PIF-0

74.0

E12, 17, 126

 

Yes(Yes)115, 126

M5 (1; 1.71)

116

139

ME53

52.6

NC127

NC127, 128

Yes129

M1 (1; 2.00), M3 (6; 19.73), M5 (1; 2.00)

117

141

EXON0

30.1

Unknown130

NC130

Yes131

M3 (1; 4.21), M5 (1; 5.36)

118

142

 

55.5

ENC17

NC101, 132

Yes(No)132, 133

M1 (8; 21.22), M2 (15; 47.90), M3 (16; 41.06), M5(2; 4.2)

119

143

ODV-E18

10.4

E17, 22, 134

E17, 22

Yes135

M5 (1; 23.76)

120

144

ODV-EC27

33.5

ENC134

NC101

 

M3 (7; 31.03)

121

145

 

11.0

Unknown136

Unknown136

Yes(Yes)136

M5 (1; 22.11)

122

146

 

22.9

Unknown137

NC137

Yes137

M5(4; 28.36)

124

148

ODV-E56/PIF-5

41.3

E17, 138

Unknown22

Yes(Yes)139, 140

M1 (1; 5.33), M3 (5; 24.00), M5(3; 9.6)

125

149

 

12.3

   

M5 (1; 27.36)

128

153

PE38

36.1

  

Yes(Yes)141

M5 (1; 3.24)

129

154

 

8.9

   

M3 (3; 66.23), M5 (3; 67.53)

130

1

PTP

19.3

Unknown142

ENC143

Yes(Yes)142

M5 (1; 9.52)

131

2

BRO-d

40.1

   

M2 (2; 5.73)

134

5

 

12.4

Unknown144

  

M1(3; 40.37), M2(2; 32.11), M3(2; 32.11), M4(3; 40.37), M5(10; 72.38)

  1. †Proteins were reported to be envelope (E)-, envelope-and-nucleocapsid (ENC)-, and/or nucleocapsid (NC)-associated components of BV and ODV in the literature.