Table 2 Viral proteins identified in the BmNPV polyhedral matrix by a Q Exactive LC-ESI-MS/MS analysis.
BmNPV ORF | AcMNPV ORF | Protein designation | Predicted molecular mass (kDa) | Location in ODV virion†| Location in BV virion†| OB formation when deleted? (ODV embedded?) | Gel region(s) (No. of peptides identified; Coverage (%)) |
---|---|---|---|---|---|---|---|
1 | 8 | Polyhedrin | 28.8 | ENC17 | NC17 | No | M1 (18; 64.9), M2 (28; 97.55), M3 (27; 97.55), M4 (25; 97.55), M5 (23; 96.33) |
2 | 9 | P78/83 | 60.9 | Â | M2(6; 16.42), M3 (3; 9.23), M5 (1; 2.40) | ||
3 | 10 | PK1 | 32.4 | NC40 | NC40 | No41 | M3 (1; 4.36) |
4 | 11 | Â | 39.8 | Â | Â | Yes(No)42 | M1 (1; 4.12), M3 (9; 36.47) |
5 | 13 | Â | 39.3 | Â | Â | Yes(Yes)43 | M4 (1; 2.42) |
7 | 15 | EGT | 57.0 | Â | Â | Yes(Yes)44 | M5 (1; 2.77) |
8 | 16 | BV/ODV-E26 | 26.2 | ENC45 | ENC45 | Yes(Yes)44 | M5 (1; 6.11) |
9 | 17 | Â | 24.1 | Â | ENC17 | M5(2; 14.29) | |
10 | 18 | Â | 41.5 | Â | Â | Yes(Yes)47 | M1 (1; 5.06), M3 (6; 24.44) |
11 | 19 | Â | 12.5 | Â | Â | Â | M2 (1; 15.45) |
14 | 23 | F protein | 78.0 | E12 | M1 (1; 2.23) | ||
15 | 24 | PKIP | 19.4 | Â | ENC17 | No50 | M5 (7; 49.70) |
17 | 26 | Â | 14.5 | Unknown17 | Â | Â | M1 (1; 9.30), M5 (7; 25.58) |
19 | 28 | LEF-6 | 20.3 | Â | Unkown17 | Â | M5 (1; 6.36) |
22a | Â | Â | Â | Â | Â | Â | M3 (1; 20.37) |
23 | 31 | SOD | 16.3 | E17 | Â | Yes(Yes)51 | M1 (2; 19.87), M3 (2; 15.23), M5(5; 53.64) |
26 | 35 | Ubiquitin | 8.7 | Yes35 | M1 (2; 32.47), M2 (1; 20.78), M3 (3; 49.35), M5(2; 44.16) | ||
27 | 36 | PP31 | 31.5 | Â | Unknown22 | Yes(Yes)53 | M1 (1; 5.78), M2 (1; 5.42) |
29 | 38 | Â | 25.5 | Â | E54 | Â | M1 (1; 3.69), M3 (1; 4.15), M5 (1; 5.07) |
30 | 39 | P43 | 43.3 | Â | Â | Yes55 | M2 (1; 4.14), M3 (1; 4.14) |
34 | 43 | Â | 9.0 | Â | Â | M3 (1; 19.23), M5(6; 61.54) | |
36 | 45 | Â | 22.5 | Â | Â | Â | M3 (1; 5.18) |
37 | 46 | ODV-E66 | 79.2 | Â | Yes(Yes)59 | M1(10; 21.08), M2(21; 49.29), M3(17; 41.60), M5(5; 13.25) | |
38 | 47 | ETS/TRAX | 10.5 | Â | Â | Â | M5 (1; 12.36) |
40 | 51 | Â | 37.8 | ENC17 | Â | M3 (3; 13.48) | |
43 | 54 | VP1054 | 42.0 | NC17 | NC61 | M3 (2; 7.12) | |
47 | 58/59 | ChaB | 37.8 | NC17 | Â | M5(4; 46.2) | |
48 | 60 | Â | 9.7 | Â | Â | Â | M1 (1; 12.05), M3 (1; 12.05), M5(2; 13.25) |
49 | 61 | FP25K | 25.3 | M5 (1; 8.88) | |||
51 | 63 | Â | 18.5 | Â | E69 | Â | M4 (1; 5.81) |
53 | 65 | DNAPOL | 114.4 | Unknown17 | Â | No70 | M1 (1; 2.64) |
54 | 66 | Â | 93.3 | NC17 | Yes72 | M1(8; 15.16), M2(2; 3.85) | |
55 | 67 | LEF-3 | 44.9 | Unknown16 | Â | Â | M2(1; 3.90), M3(6; 20.26), M4(1; 3.38), M5 (1; 2.60) |
57 | 69 | Â | 30.4 | Â | Â | Yes(Yes)73 | M5 (1; 7.63) |
59 | 73 | Â | 11.5 | Â | Unknown22 | Yes(Yes)74 | M5(4; 58.59) |
60 | 74 | Â | 31.0 | Unknown12 | E17 | Â | M3 (1; 3.36) |
61 | 75 | Â | 15.5 | E17 | ENC17 | Â | M5 (5; 35.34) |
62 | 76 | Â | 9.6 | E75 | E75 | Yes(No)76 | M1 (1; 15.29), M5(2; 16.47) |
63 | 77 | VLF-1 | 44.3 | ENC17 | NC77 | M1 (6; 17.41), M2 (3; 9.50), M3 (8; 26.65), M5(4; 12.93) | |
65 | 79 | Â | 12.2 | Unknown12 | Â | Yes(Yes)79 | M5 (2; 17.31) |
66 | 80 | GP41 | 44.9 | ENC17 | ENC17 | Yes62 | M1 (12; 41.69), M2 (6; 17.12), M3 (15; 59.31), M4(1; 6.20), M5(2; 9.18) |
67 | 81 | Â | 27.0 | Unknown17 | Â | Yes(No)80 | M2 (1; 4.27), M5 (1; 5.56) |
68 | 82 | TLP | 20.1 | Â | E81 | Yes(Yes)81 | M2 (2; 25.41), M4(1; 4.42) |
69 | 83 | Â | 95.8 | Â | M1 (2; 4.53), M2 (2; 4.77), M5 (1; 2.26) | ||
70 | 87 | VP15 | 15.1 | Â | Â | Â | M1 (1; 17.46), M2 (1; 17.46), M3 (1; 17.46) |
71 | 88 | CG30 | 30.7 | Unknown12 | Â | M1 (1; 5.24), M3 (1; 6.37) | |
72 | 89 | VP39 | 39.3 | Â | M1 (1; 4.00), M3 (4; 20.29), M5 (1; 4.00) | ||
75 | 92 | P33 | 30.9 | ENC89 | Yes(No and singly enveloped nucleocapsid)88 | M1(7; 22.01), M2(7; 32.05), M3(8; 28.96), M4(16; 77.22), M5(14; 62.93) | |
76 | 93 | Â | Â | NC90 | ENC90 | Yes(No)90 | M5(4; 32.92) |
77 | 94 | ODV-E25 | 25.6 | Yes(No)92 | M1 (2; 15.35), M2 (1; 7.89), M3 (3; 28.07), M5(10; 60.96) | ||
78 | 95 | DNA helicase/P143 | 143.6 | Â | Â | M2 (1; 0.65), M5 (1; 1.15) | |
79 | 96 | PIF-4 | 21.0 | E95 | Yes95 | M5 (1; 8.79) | |
80 | Â | BRO-b | 27.5 | Â | Â | Â | M2 (1; 4.18) |
81 | Â | BRO-c | 35.9 | Â | Â | Â | M4(1; 2.83), M5 (2; 7.55) |
82 | 98 | 38 K | 38.0 | Yes(No)97 | M3 (3; 11.88) | ||
84 | 100 | P6.9 | 8.1 | Yes(No)99 | M1 (1; 12.31) | ||
85 | 101 | BV/ODV-C42 | 41.6 | Â | M3 (5; 21.55), M5 (1; 1.93) | ||
87 | 103 | P48 | 45.4 | Â | ENC17 | Yes(No)102 | M1 (5; 12.66), M2 (3; 10.34), M3 (12; 39.02), M5 (2; 5.17) |
88 | 104 | VP80 | 79.9 | Â | M1 (9; 19.65), M2 (10; 23.12), M3 (7; 15.75) | ||
91 | 108 | Â | 11.8 | Â | Yes(Yes)106 | M3 (1; 27.62) | |
92 | 109 | ODV-EC43 | 45.0 | ENC108 | M1 (5; 18.41), M3 (14; 53.96), M5(4; 12.79) | ||
92a | Â | Â | 6.8 | Â | Â | Â | M2 (1; 47.46) |
93 | 111 | Â | 8.2 | Â | Â | Â | M1 (1; 20.09), M5 (1; 14.93) |
94 | 114 | Â | 49.4 | Unknown22 | Yes(Yes)112 | M1 (3; 10.14), M3 (11; 39.86), M5(3; 12.74) | |
95 | 115 | PIF-3 | 23.0 | Â | Â | ||
95a | 116 | Â | Â | Â | Â | Â | M1 (1; 25.00) |
98 | 120 | Â | 9.5 | Â | Â | Â | M5 (1; 13.41) |
100 | 123 | PK2 | 26.0 | Â | Â | Â | M5 (6; 28.89) |
104 | 127 | V-cath | 36.9 | Â | ENC116 | Â | M1 (1; 3.10), M5 (4; 17.96) |
106 | 129 | P24 | 21.8 | Â | M1 (6; 37.44), M2 (5; 46.15), M3 (8; 58.97), M4 (2; 11.28), M5 (15; 73.33) | ||
107 | 130 | GP16 | 12.1 | Â | Â | Yes(Yes)118 | M5 (1; 10.38) |
108 | 131 | PEP | 35.4 | NC17 | NC17 | Yes(Yes)119 | M1 (5; 16.51), M2 (2; 9.52), M3 (7; 32.70), M4 (3; 19.68), M5(5; 26.03) |
109 | 132 | Â | 25.2 | NC120 | NC120 | Yes(Few)120 | M4 (1; 4.09) |
110 | 133 | ALK-EXO | 48.5 | Unknown12 | Â | Â | M3 (1; 1.67) |
110a | Â | Â | Â | Â | Â | Â | M3 (1; 28.07) |
111 | Â | Â | Â | Â | Â | Yes(Yes)121 | M3 (1; 20.00) |
112 | 135 | P35 | 34.5 | Â | Â | M3 (6; 24.08), M5(2; 11.04) | |
114 | 137 | P10 | 7.5 | Â | Â | M5 (1; 27.14) | |
115 | 138 | P74/PIF-0 | 74.0 | Â | M5 (1; 1.71) | ||
116 | 139 | ME53 | 52.6 | NC127 | Yes129 | M1 (1; 2.00), M3 (6; 19.73), M5 (1; 2.00) | |
117 | 141 | EXON0 | 30.1 | Unknown130 | NC130 | Yes131 | M3 (1; 4.21), M5 (1; 5.36) |
118 | 142 | Â | 55.5 | ENC17 | M1 (8; 21.22), M2 (15; 47.90), M3 (16; 41.06), M5(2; 4.2) | ||
119 | 143 | ODV-E18 | 10.4 | Yes135 | M5 (1; 23.76) | ||
120 | 144 | ODV-EC27 | 33.5 | ENC134 | NC101 | Â | M3 (7; 31.03) |
121 | 145 | Â | 11.0 | Unknown136 | Unknown136 | Yes(Yes)136 | M5 (1; 22.11) |
122 | 146 | Â | 22.9 | Unknown137 | NC137 | Yes137 | M5(4; 28.36) |
124 | 148 | ODV-E56/PIF-5 | 41.3 | Unknown22 | M1 (1; 5.33), M3 (5; 24.00), M5(3; 9.6) | ||
125 | 149 | Â | 12.3 | Â | Â | Â | M5 (1; 27.36) |
128 | 153 | PE38 | 36.1 | Â | Â | Yes(Yes)141 | M5 (1; 3.24) |
129 | 154 | Â | 8.9 | Â | Â | Â | M3 (3; 66.23), M5 (3; 67.53) |
130 | 1 | PTP | 19.3 | Unknown142 | ENC143 | Yes(Yes)142 | M5 (1; 9.52) |
131 | 2 | BRO-d | 40.1 | Â | Â | Â | M2 (2; 5.73) |
134 | 5 | Â | 12.4 | Unknown144 | Â | Â | M1(3; 40.37), M2(2; 32.11), M3(2; 32.11), M4(3; 40.37), M5(10; 72.38) |