Figure 2
From: Dimerization in tailoring uptake efficacy of the HSV-1 derived membranotropic peptide gH625
Inner monolayer in DOPC/Chol 60/40 (mol/mol) (A) and DOPG/Chol 60/40 (mol/mol) (B). Two populations of LUVs, one labelled with 0.6 mol% of NBD (donor) and Rho (acceptor) and one unlabelled were mixed; the NBD located on the outer monolayer of LUVs, was completely reduced by treatment with sodium dithionite (100 mM) and the variation in NBD fluorescence due to membrane fusion of inner monolayer was determined. Lipid concentration is 0.1 mM. Percentage of inner monolayer fusion as a function of the peptide/lipid ratio is reported and each trace represents an average of three independent experiments.
