Figure 1 | Scientific Reports

Figure 1

From: Targeted blockade of TGF-β and IL-6/JAK2/STAT3 pathways inhibits lung cancer growth promoted by bone marrow-derived myofibroblasts

Figure 1

Lung cancer cells promoted MFs proliferation and cytokine secretion via TGF-β. MFs were cultured in normal medium, CMT-167-conditioned medium (CMT-CM) or LLC-conditioned medium (LLC-CM) and the proliferation of MFs was measured by CCK-8 at 0 h, 24 h and 48 h (A,B). Cancer-CM enhanced MF proliferation. In a second set of experiments, MFs were cultured alone, with mrTGF-β (2 ng/mL), with caner condition-medium or co-cultured with CMT/LLC cells. mIL-6 concentration in supernatant was measured by ELISA (C–F) and mIL-6 protein in MFs was assessed by Western blots (G,H). mrTGF-β, lung cancer cells and cancer-CM enhanced expression of mIL-6 in MFs. n = 6 wells/group; *Compared to MF group, p < 0.05; #Compared to NM group, p < 0.05.

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