Figure 2 | Scientific Reports

Figure 2

From: Targeted blockade of TGF-β and IL-6/JAK2/STAT3 pathways inhibits lung cancer growth promoted by bone marrow-derived myofibroblasts

Figure 2

MFs promoted lung cancer cell proliferation and cytokine production via IL-6. CMT/LLC cells were cultured in normal medium or MF-CM and the proliferation of CMT/LLC cells was measured by CCK-8 at 0 h, 24 h and 48 h (A,B). MF-CM was able to promote cancer cell proliferation. CMT/LLC cells were cultured alone, with mrIL-6 (20 ng/mL) or with MF-CM, or co-cultured with MFs. mTGF-β concentration in supernatant was measured by ELISA (C–F). mTGF-β protein levels were measured by Western blots (G,H). mrIL-6, MFs and MF-CM were both able to enhance the mTGF-β expression in lung cancer cells. n = 6 wells/group; *Compared to CMT or LLC group, p < 0.05; #Compared to NM group, p < 0.05.

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