Figure 1
From: CUBIC pathology: three-dimensional imaging for pathological diagnosis
Tissue clearing of various human organs with CUBIC. (a) Schematic diagram of clearing protocol for human organs. IHC, immunohistochemistry. (b) Gross and microscopic images of human or mouse lung and lymph nodes. After gross image acquisition at the indicated time points, lung and lymph node tissues were washed with PBS, followed by paraffin embedding, sectioning, and H&E staining. (c) Representative images of immunohistochemistry of human lung and lymph node tissues after clearing by CUBIC. Serial sections prepared from lung or lymph node tissues which were histologically evaluated in (b) were immunostained with anti-cytokeratin (epithelial cell marker), anti-α-SMA (smooth muscle cell marker), or anti-CD68 (monocyte/macrophage marker) antibodies (lungs), or immunostained with anti-CD79a (B-cell marker), anti-CD3 (T-cell marker), or anti-CD68 (monocyte/macrophage marker) antibodies (lymph nodes). DAB, 3,3′-diaminobenzidine.
