Figure 2
From: CUBIC pathology: three-dimensional imaging for pathological diagnosis
3D imaging of various human organs with CUBIC. (a) Schematic diagram of clearing and immunostaining protocol for human lungs, lymph nodes, and peripheral arteries. IHC, immunohistochemistry. (b,c) The reconstructed 3D images and orthogonal section images of human lung tissue blocks stained with Alexa Fluor 647-conjugated anti-α-SMA antibody and SYTO 16, with or without clearing by CUBIC. Images were obtained by confocal microscopy (z-stack: 1 μm/slice). (d) The reconstructed 3D images of SYTO 16-stained human lung tissue block, SYTO 16-stained half-cut human mesenteric lymph node, SYTO 16-stained peripheral branch of coronary artery which was dissected from a human heart, and SYTO 16- and Alexa Fluor 594-labeled phalloidin-stained peripheral arteries in the surface of human cerebral cortex block. Images were obtained by light-sheet fluorescence microscopy (LSFM) (z-stack: 5 μm/slice).
