Figure 3
From: CUBIC pathology: three-dimensional imaging for pathological diagnosis
Tissue clearing and 3D imaging of pathological specimens with CUBIC. (a) Schematic diagram of clearing and Congo Red staining protocol for human tissue blocks of lung amyloidosis. (b) Representative histopathological images of this case of lung amyloidosis, which was obtained from glass slides generated for post-mortem diagnosis. H&E staining (upper) and Congo Red staining (lower) show pink-colored amyloid deposition in blood vessel walls in lung. (c) Gross images of amyloidosis patient-derived and normal lung tissue blocks after Congo red staining or PBS soaking. An image of an amyloidosis patient-derived lung tissue block (upper, left) shows that arteries are macroscopically visible in red. (d) The reconstructed 3D images of human lung tissue block stained with Congo Red, showing that amyloid deposition in artery wall in lung. The images were obtained by LSFM (z-stack: 5 μm/slice) (upper panels) or confocal microscopy (z-stack: 1 μm/slice) (lower panels).
