Figure 3
Thrombin-enhanced CXCL8 expression through PAR1 receptor. (A) NP cells were transfected with siRNA against PAR1, PAR2, PAR3, or PAR4, or the non-targeting control, for 24 h followed by treatment with thrombin (10 U/mL) for another 24 h, and then CXCL8 protein levels were determined by ELISA. Each bar represents the mean ± S.E.M. from at least three independent experiments performed in duplicate. (B) NP cells were transfected with siRNA against PAR1, PAR2, PAR3, or PAR4 for 24 h. (C) NP cells were treated with various concentrations of thrombin for 4–6 h. The gene expression of PAR1 receptor was determined by real time-PCR. Each bar represents the mean ± S.E.M. from three independent experiments performed in duplicate. *p < 0.05 compared with the control group. # p < 0.05 compared with the thrombin-treated group.
