Figure 2
RT-qPCR validation of microarray data from DRP treated plants. Highly expressed unique genes under DRP stress from microarray were selected and corresponding transcript accumulation under D, P and DRP stress treatment were compared by RT-qPCR analysis. Fold change in expression levels relative to the control samples were normalized to AtACTIN2 gene expression. RT-qPCR based quantification was performed with three biological replicates (two technical replicates each). Graph represents the relative fold change values (compared to control or mock treatment) in the expression of genes encoding for AtACS11 (A) AtAPK4 (B) AT1G69570, a DOF-type Zinc finger type protein (C) AT3G49340, a cysteine proteinases superfamily protein (D) and AT5G44570, an unknown protein (E). Represented data are the average of three biological replicates from one experiment, and error bars show standard error of the mean (SEM). Significance was calculated using Student’s t-test where * and ‡ symbol shows significance at p < 0.05 over drought and pathogen stress respectively. Fold change values in gene expression obtained from microarray and RT-qPCR analysis under DRP stress treatments were compared with scatter plot (F). Gene names and descriptions for the gene IDs presented in the figure are provided in Supplementary File S2. Details of primers used in the study are provided in Supplementary File S4.
