Figure 6
Microscopy of HT29 (a,b,c,d,e,f) and A549 (g,h,i,j,k,l) cells treated with CPT-CEF to show changes in cell morphology. Cells were exposed to the IC50 concentration of CPT-CEF and viewed at 24 and 48 h. Panel a and g (Untreated cells at 24 h), b and h (IC50 treated cells for 24 h), c and i (Untreated cells at 48 h), and d and j (IC50 treated cells for 48 h). Under phase contrast microscopy, cell morphology changes in treated cells are clearly visible in comparison to untreated cells; the cell membrane is evidently damaged. Panels’ e and f show fluorescent micrographs of AO/PI double-stained human colorectal cancer cells (HT29). Cells were exposed to the IC50 concentration of CPT-CEF (panel f and i) or vehicle (panel e and k) for 48 h. Cell apoptosis was assayed by AO/PI staining to detect chromosomal condensation (CC), late stage apoptosis (LS), necrosis (N), and membrane blebbing (MB), as shown in the micrograph. Microscope magnification × 100 and scale bar of 10 µm (HT29) and scale bar of 100 µm (A549) were applied for the images.
