Figure 7

Annexin V/PI assay showing the apoptosis-inducing effect of CPT-CEF. HT29 cells were treated with various concentrations of CPT-CEF: ¼ IC₅₀ (panel b), ½IC₅₀ (panel c), IC₅₀ (panel d), or untreated (panel a) for 48 h, and viability was assessed via Annexin V/PI assay and flow cytometry.