Figure 3
From: Ribosomal transcription is regulated by PGC-1alpha and disturbed in Huntington’s disease
PGC-1α stimulates rDNA transcription in vitro and in vivo. (3a) PGC-1α-KO tissue (BAT) show reduced rDNA transcription. QPCR with m45S-primer from wt (orange) and ko (red) BAT cDNA. N = 3, **p = 0.0037; one way ANOVA; column: mean ± SD. (3b) Promoter occupancy of the initiation complex in KO tissue in comparison to WT. QPCR and Western blots of ChIPs from wt (orange) and ko (red) BAT chromatin N = 3, **p = 0.0013; ****p < 0.0001; one way ANOVA; column: mean ± SD. (Full-length blots see supplement). (3c) RNA polymerase I transcription analysis of WT and PGC-1α-KO tissues and cultivated mouse fibroblasts. qPCR with m45S-primer. N = 3; *p = 0.0134, ***p = 0.0006; one way ANOVA; column: mean ± SD. (3d) Treatment with 10 µM resveratrol for 3h increases transcription of the 47 S rRNA in cell culture. HEK-cells kept under 3% pO2, transfected with flag-tagged PGC-1α and pUC18. The panels are a representative of four independent experiments; **p = 0.0044, one way ANOVA; column: mean ± SD. (3e) PGC-1α-expression after 3 h treatment with resveratrol (10 µM). HEK-cells kept under 3% pO2, transfected with flag-tagged PGC-1α and pUC18. ****p < 0.0001; one way ANOVA; column: mean ± SD.
