Figure 3

Waveform analysis of neuronal calcium transients. Raw Δ fluorescence traces from neuronal cultures (DIV8–DIV15) were analyzed using the Matlab Signal Processing Toolbox, to extract amplitude (A), pulse width (B), frequency (C), fall time (D), and rise time (E) for each ROI. (F) Synchronization was assessed by calculation of the Pearson correlation coefficient. Plots show averaged values from >16 acquisitions at each time point taken from 4 independent experiments. Solid lines represent linear regressions with 95% CI (dotted line). (G–I) Calcium traces were acquired as above from DIV14 cortical neuron cultures. (G) Spatial coefficient of variance (CV) for each of the computed waveform parameters was determined by comparing average values across multiple optical fields within the same well (n = 6 fields per well). (H) Temporal CV was determined for each waveform parameter by comparing the average values across serial acquisitions from the same optical field (n = 6 serial captures). (I) The coefficient of variation among all ROIs from a given optical field for each of the indicated metrics was calculated for n = 74 separate acquisitions (each dot represents one acquisition). Data are representative of 2 independent neural cultures.