Figure 5

Cytokine treatment alters neuronal firing kinetics and increases network synchronization. Representative raster plots are shown for DIV 8 (A,G) or DIV 12 (D,J) cortical neurons treated for 24 hours with 100 ng /mL TNF-α, 100 ng/ mL IFN-γ, 10 ng/mL IL-1β or vehicle alone (PBS). After 24 hours of cytokine stimulation, cells were treated acutely with 120 nM midazolam (G–I) or 10 μM GABA (J–L) prior to signal acquisition. Average amplitude and Pearson correlation coefficient values are plotted for DIV 8 neurons prior to (B,C) and after treatment with 120 nM midazolam (H,I) as well as for DIV 12 neurons prior to (E,F) and after treatment with 10 uM GABA (K,L) for all ROIs across 6 wells for each of the indicated conditions. Box plots show median (line), average (+), and interquartile range; error bars represent 10–90% range with outliers shown. Data are representative of 2–3 independent experiments.