Figure 2
From: Bioenergetic Adaptations in Chemoresistant Ovarian Cancer Cells
Bioenergetics organization of chemosensitive and chemoresistant ovarian cancer cells. (A) Oxygen consumption rate (OCR) was measured in chemosensitive A2780 and resistant C200 isogenic cell lines and (F) chemosensitive PEO1 and resistant PEO4 isogenic cell lines by a cell mitochondrial stress test when challenged by (1) oligomycin (an ATP synthase inhibitor), (2) FCCP (an electron transport chain uncoupler) and (3) rotenone (an inhibitor of electron transport chain). (B,G) Basal OCR represents the mitochondrial respiration at resting state, the maximum respiration indicates the cellular reaction to an increased ATP demand using FCCP mitochondrial uncoupler, and respiratory reserve and ATP-linked respiration indicate greater capacity to utilize mitochondria. These were calculated as described in the Materials and Methods. ***p < 0.001, **p < 0.01 chemoresistant cells (C200 and PEO4 compared to respective chemosensitive cells [A2780 and PEO1]). Extracellular acidification rate (ECAR) was measured in (C) chemosensitive A2780 and chemoresistant C200 isogenic cell lines and (H) chemosensitive PEO1 and resistant PEO4 isogenic cell lines when challenged by (1) glucose (fuel for glycolysis), (2) oligomycin (an ATP synthase blocker) and (3) 2DG (an inhibitor of glycolysis). (D,I) Basal ECAR represents the glycolysis measure at resting state, glycolytic capacity represents the total ability of the cell to perform glycolysis (resting state and enforced combined) and glycolytic reserve defines the capacity to increase glycolysis when mitochondria is compromised, were calculated as described in the methods. ***p < 0.001, **p < 0.01, *p < 0.05 chemoresistant cells (C200 and PEO4 compared to respective chemosensitive cells [A2780 and PEO1]). (E,J) Ratio of OCR: ECAR quadrant showing the bioenergetics phenotype of each cell line. All Seahorse experiments (Seahorse Biosciences, Boston, MA) were performed for a minimum of 5 times in triplicates. Abbreviations: ATP: adenosine triphosphatase; 2DG: 2-deoxyglucose; FCCP: carbonylcyanide-p-trifluoromethoxyphenyl hydrazine.
